Figure 1.

Triacylglycerol accumulation in undifferentiated feline liver organoids. Organoids from 6 different cats were incubated in the absence (no‐free fatty acids [FFA]) or presence of additional fatty acids and in the absence or presence of 5‐aminoimidazole‐4‐carboxamide 1‐β‐D‐ribofuranoside (AICAR, an adenosine monophosphate kinase activator, 2mM), T863 (diacylglycerol O‐acyltransferase 1 [DGAT1] inhibitor, 20 μM) or a combination of a T863 (DGAT1 inhibitor, 20 μM) and PF 06424439 (DGAT2 inhibitor, 50 μM). *** P = .003, **** P < .001. 100% represents TAG accumulation in the presence of additional fatty acids and dimethyl sulfoxide as vehicle control. Each dot represents the average of a culture duplicate from 1 cat