Figure 2.

Presence of lipid droplets in feline liver organoids after inducing lipid accumulation. Organoids were incubated in the absence (no‐free fatty acids [FFA]) or presence (FFA) of additional fatty acids and in the absence (dimethyl sulfoxide) or presence of 5‐aminoimidazole‐4‐carboxamide 1‐β‐D‐ribofuranoside (AICAR, an adenosine monophosphate kinase activator, 2mM), T863 (diacylglycerol O‐acyltransferase 1 [DGAT1] inhibitor, 20 μM), or a combination of a T863 (DGAT1 inhibitor, 20 μM) and PF 06424439 (DGAT2 inhibitor, 50 μM). Fluorescence microscopic images are from 2 different cats (cat 1 and cat 2). Blue = Hoechst, green = LD540