Figure 3.

Triacylglycerol accumulation in differentiated feline liver organoids. A and B, Organoids were cultured in expansion medium (EM) or differentiation medium (DM), and relative gene expression of stem cell (leucine‐rich repeat‐containing G protein‐coupled receptor 5) and hepatocyte (TTR and CYP3a132) marker genes (A) and intracellular TAG concentrations (B) was measured. C, TAG accumulation in differentiated feline liver organoids after administration of drugs. Organoids were incubated in the absence (no‐free fatty acids [FFA]) or presence of additional fatty acids and in the absence or presence of 5‐aminoimidazole‐4‐carboxamide 1‐β‐D‐ribofuranoside (AICAR, an adenosine monophosphate kinase activator, 2mM), T863 (diacylglycerol O‐acyltransferase 1 [DGAT1] inhibitor, 20 μM), or a combination of a T863 (DGAT1 inhibitor, 20 μM) and PF 06424439 (DGAT2 inhibitor, 50 μM). 100% represents TAG accumulation in the presence of additional fatty acids, without drugs. **P = .01 ***P = .006, ****P < .001