Table 2.
Immunological reagents for cell identification and functional assessment in dogs with cancer.
| Molecule | Cellular expression | Usage |
|---|---|---|
| CD3 | T cells | FC, IHC |
| CD5 | T cells | FC |
| CD4 | Th subset, neutrophils | FC, IHC |
| CD8 | Tc subset | FC, IHC |
| CD9 | Myeloid cells, T cells | FC |
| CD11a | Leukocytes, memory T cells | FC |
| CD11b | Myeloid cells | FC, IHC |
| CD11c | DC, some macrophages | FC, IHC |
| CD14 | Monocytes, some neutrophils | FC |
| CD18 | Myeloid cells, MH | FC, IHC |
| CD19, CD20, CD21 | B cells, lymphoma | FC |
| CD25 | Activated T cells, Tregs | FC |
| CD31 | Endothelial cells | IHC |
| CD34 | Hematopoietic stem cells | FC |
| CD40 | APC | FC |
| CD45 | All hematopoietic cells | FC |
| CD61 | Platelets | FC |
| CD79a | Pre-B cell | IHC |
| CD86 | APC | FC |
| MHCII | T cells, APC | FC, IHC |
| FoxP3 | Regulatory T cells | FC, IHC |
| Granzyme B | T cells | FC, IHC |
| TNF-a | T cells, APC | FC, IHC |
| IFN-g | T cells, NK cells | FC, IHC |
| EOMES | T cell (exhausted; memory) | FC |
| Tim-3 | T cell (exhausted) | FC |
| PD-1 | T cell (exhausted); also recently activated | FC |
| PD-L1 | Monocyte, macrophage, DC | FC, IHC |
| Ki67 | Proliferating cells | FC, IHC |