Skip to main content
. 2020 Jan 17;10:3002. doi: 10.3389/fimmu.2019.03002

Figure 6.

Figure 6

The expression of NGK2A on tumor-infiltrating CD8+ T cells is TCR strength-dependent. The single-cell suspension of paired normal tissue and tumor stimulated for 72 h with nothing or 1/4 or 1/2 or recommended dose concentration of CD3 and CD28. (A) Bar diagram shows the NKG2A expression change. N (healthy normal tissue adjacent to the tumor, n = 7) and T (tumor, n = 7). Data are shown as the mean ± SEM; n = 6; *p < 0.05; **p < 0.01. (B) Representative flow cytometric analysis of the expression of NKG2A and intracellular IFN-γ produced by CD8+ T cells. (C) Bar diagram summarizes the levels of intracellular IFN-γ produced by CD8+ T cells and CD8+NKG2A+ T cells. Data are shown as the mean ± SEM; n = 6; *p < 0.05. (D) Representative flow cytometric analysis of the expression of NKG2A and intracellular granzyme B produced by CD8+ T cells. (E) Bar diagram summarizes the levels of intracellular granzyme B produced by CD8+ T cells and CD8+NKG2A+ T cells. Data are shown as the mean ± SEM; n = 6; **p < 0.01; ***p < 0.001. (F) Representative flow cytometric analysis of the expression of NKG2A and PD-1in CD8+ T cells. (G) Bar diagram summarizes the levels of PD-1 in CD8+ T cells and CD8+NKG2A+ T cells. Data are shown as the mean ± SEM; n = 6; *p < 0.05.