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. 2020 Jan 17;10:1529. doi: 10.3389/fphar.2019.01529

Figure 3.

Figure 3

β-Asarone induction ameliorated Aβ pathology and synaptic damage in Aβ1-42 PC12 cell model of AD. PC12 cells were treated with 7 μM Aβ1-42 for 12 h; then we cultured the model cells with β-asarone 24, 36, 72 μM or donepezil 9.6 μM for another 12 h. (A) IF microscopy and data analysis of BACE1 (B), APP (C), PS1 (D), and SYN-1 (E) showed: compared with normal group, APP, PS1, and BACE1 all increased significantly. β-asarone could decrease the expression of BACE1, APP, and PS1; donepezil was effective in reducing the expression of BACE1 and PS1. SYN-1 were all improved after culture with them. (Compared with normal group, # P < 0.05, ## P < 0.01, ### P < 0.001; compared with model group, *P < 0.05, **P < 0.01, ***P < 0.001; one-away ANOVA, n = 6). (F) Western blot data showed the effects of β-asarone on the expression levels of proteins involved, APP, SYN-1, BACE1, Aβ, and PS1. β-asarone suppressed the expression of APP, PS1, Aβ, and BACE1 and promoted SYN1. (Compared with model group, *P < 0.05, **P < 0.01, ***P < 0.001; one-away ANOVA, n = 6).

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