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. 2020 Jan 17;10:1529. doi: 10.3389/fphar.2019.01529

Figure 5.

Figure 5

β-asarone promoted autophagy- and mitophagy-related proteins in Aβ1-42 PC12 cell model of AD. PC12 cell was treated with 7 μM Aβ1-42 for 12 h; then we cultured the model cells with β-asarone 36 μM, A3 1 μM, CSA 20 μM, and 3MA 200 μM respectively for another 12 h. (A) Immunofluorescence (IF) microscopy and data analysis of P62 (B), LC3 (C), and BECN (D) showed: compared with normal group, P62 increased while LC3 and BECN decreased; β-asarone could improve the expression of LC3 and BECN. (Compared with normal group, # P < 0.05, ## P < 0.01, ### P < 0.001; compared with model group, *P < 0.05, **P < 0.01; one-away ANOVA, n = 6). (E) Western blot data showed the effects of β-asarone on the expression levels of proteins involved P62, LC3, BECN, PINK1, and Parkin. β-asarone showed effect on reducing P62 and improving PINK1. (Compared with model group, # P < 0.05, ## P < 0.01; *P < 0.05, **P < 0.01; & P < 0.05; one-away ANOVA, n = 6).