Table 3.
Strength-of-evidence summary. For each measured endpoint in each data stream samples were classified as “similar” to the reference sample - GBE 1 (green color, value of 1), “different” from the reference (red color, value of −1) or “maybe similar” to the reference (yellow color, value of 0).
| Data stream Endpoint | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Untargeted chemistry Unhydrolyzed samples | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | 0 | 0 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 |
| Untargeted chemistry Hydrolyzed samples | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 |
| Untargeted chemistry score | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −0.5 | −0.5 | 0 | 0 | −0.5 | −0.5 | −0.5 | −1 | −0.5 | −0.5 | −0.5 | 0 | 1 | 1 | 1 | 1 | 1 | 1 |
| Targeted chemistry Unhydrolyzed | −1 | −1 | −1 | 0 | 0 | −1 | −1 | −1 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | 1 | 1 | 1 | 1 |
| 1 | ||||||||||||||||||||||||||
| Targeted chemistry Hydrolyzed | −1 | −1 | −1 | 1 | 1 | −1 | −1 | −1 | 0 | 0 | 1 | 0 | −1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | ||||||||||||||||||||||||||
| Targeted chemistry score | −1 | −1 | −1 | 0.5 | 0.5 | −1 | −1 | −1 | 0 | 0 | 0.5 | 0 | −1 | 0.5 | 0 | 0.5 | 0.5 | 0.5 | 0 | 0.5 | 0.5 | −0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| SC-PHH gene expression | −1 | −1 | −1 | 1 | 1 | −1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
| SC-PHH score | −1 | −1 | −1 | 1 | 1 | −1 | −1 | −1 | 1 | 1 | 1 | 1 | −1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
| Attagene Cis-factorial assay | −1 | −1 | −1 | 1 | 1 | −1 | −1 | −1 | 1 | 0 | 0 | 0 | −1 | −1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | −1 | 1 | 1 | 1 | 1 |
| Attagene Trans-factorial assay | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | 1 | 1 |
| Attagene GPCR assay | 0 | 0 | 0 | −1 | −1 | 0 | 0 | 0 | −1 | 1 | 1 | 1 | 1 | 1 | −1 | −1 | −1 | −1 | −1 | 1 | 1 | 0 | −1 | 0 | 0 | 0 |
| Attagene score | −0.3 | −0.3 | −0.3 | 0.0 | 0.0 | −0.3 | −0.3 | −0.3 | 0.0 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | −0.3 | −0.3 | 0.0 | 0.0 | −0.3 | 0.3 | 0.3 | −0.3 | −0.3 | 0.3 | 0.7 | 0.7 |
| In vivo 5-day studies | −1 | 1 | −1 | 1 | ||||||||||||||||||||||
| In vivo score | −1 | 1 | −1 | 1 | ||||||||||||||||||||||
| Overall similarity score | −0.8 | −0.8 | −0.8 | 0.1 | 0.1 | −0.8 | −0.9 | −0.8 | 0.1 | 0.2 | 0.5 | 0.3 | −0.5 | 0.5 | 0.0 | −0.2 | 0.3 | 0.3 | 0.0 | 0.6 | 0.7 | 0.3 | 0.3 | 0.7 | 0.8 | 0.8 |
Abbreviations: SC-PHH (Sandwich cultures of primary human hepatocytes); GPCR (G-protein coupled receptor)
Samples containing a single peak or no discernable peaks were excluded from multifactorial analysis of samples and were categorized as “different” from the reference GBE.
Sample U was included in two separate targeted chemistry analyses, resulting in two data sets for the sample. These runs resulted in a determination of “maybe similar” and “similar” to the reference. Other samples were run twice in various assays, but did not result in any differences in categorization, so a single value was included for simplicity.