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. 2020 Jan 23;51:6. doi: 10.1186/s13567-020-0735-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Humoral immune responses. Five-week old female BALB/c mice were immunized with PBS, vector-only strain, and Salmonella secreting Brucella L7/L12. Humoral responses in serum and vaginal washes were measured on 14th day post-immunization. A Serum IgG response, IgA response in vaginal washes, and serum IgG1 and IgG2a were demonstrated. *Indicates significant difference compared to PBS control (P < 0.05). Probability values were depicted. B The IgG responses at 21st day post-immunization were measured against purified L7/L12 antigen, Brucella whole cell lysate and Salmonella whole cell lysate were conducted. ***Indicates the significant difference against the PBS control (P < 0.05).