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. 2020 Jan 23;11:36. doi: 10.1186/s13287-020-1563-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Mesenchymal stem cell (MSC)-derived exosomes were identified and internalized by H9C2 cells. A1, A2 Analysis of the concentration and diameters of MSC-derived exosomes using nanoparticle trafficking. B Western blot analysis of the exosome surface markers TSG101 and CD63 (MSC lysis was used as control). C H9C2 cells internalized labeled MSC-derived exosomes after a 12-h co-incubation; unlabeled exosomes were used as control. D–E Labeled exosomes but not dye alone were readily taken up by H9C2 cells. Statistics calculated based on the results of three repetitions of each experiment