Fig. 2.

Hypoxia induced apoptosis and altered expression of apoptosis-related protein in H9C2 cells cultured for 0, 12, 24, 48, or 72 h under hypoxic conditions. A1–A3 The expression of HIF-1α gene and protein was determined by real-time PCR and western blotting, respectively. B1, B2 The level of apoptosis was determined by flow cytometry. C1–C3 Expression levels of AKT, Bcl-2, and caspase-3 genes were determined by real-time PCR. D1–D5 Expression levels of AKT, p-AKT, Bcl-2, and caspase-3 were determined by western blotting. ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001; 0-h timepoint was used as control. HIF-1α, hypoxia inducible factor-1α; p-AKT/AKT, phosphorylated protein kinase B; Bcl-2, B-cell lymphoma-2. Statistics calculated based on the results of three repetitions of each experiment