Fig. 5.

A transfected miR-144 inhibitor alters H9C2 cell apoptosis and changes p-AKT/AKT expression levels in hypoxic conditions. A Validation of transfected miR-144 inhibitor along with the inhibitor control by real-time PCR. ^###P < 0.001 vs. inhibitor control. B1–B3 H9C2 cells transfected with the miR-144 inhibitor had increased apoptosis in the induction of hypoxia. ^###P < 0.001 vs. normoxia; ^##P < 0.01 vs. normoxia; ^***P < 0.001 vs. hypoxia+ inhibitor control. C1–C3 H9C2 cells transfected with the miR-144 inhibitor had altered p-AKT/AKT expression levels in hypoxic conditions. ^###P < 0.001 vs. normoxia; ^***P < 0.001 vs. hypoxia+ inhibitor control. P-AKT/AKT, phosphorylated protein kinase B. Statistics calculated based on the results of three repetitions of each experiment