Fig. 2.

Stimulation of signaling pathways by LPS and inhibition by TAK-242 in synovial fibroblasts. a Time course of signaling pathways activated by LPS. b Confocal image of NF-κB location in the nucleus and cytoplasm. TAK-242 inhibited the migration of NF-κB into the nucleus from the cytoplasm. c Western blot of nuclear protein. Protein levels of NF-κB and AP-1 in the nucleus were detected by western blot. The increased levels of NF-κB-p65 and AP-1 in response to IL-1β (10 ng/ml) or LPS (2 μg/ml) were weakly inhibited by TAK-242. The band density was measured by ImageJ program. The data shown are representative of three independent experiments, and similar results were obtained from all three