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. 2020 Jan 17;9:463. doi: 10.3389/fcimb.2019.00463

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Copyright © 2020 Li, Wu, Wang, Ma, Jia, Chen, Zhu, Liu, Yang, Zhao, Zhang, Huang, Ou, Mao, Zhang, Liu, Yu, Pan, Tian, Rehman, Chen and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prokaryotic expression of DEV ICP22 and identification of the ICP22 protein. (A) M, Protein molecular mass marker; Lane 1, untreated samples; lane 2, recombinant bacterial supernatant after IPTG induction; lane 3, the purified ICP22 protein. (B) Identification of the ICP22 protein by the prepared anti-ICP22 antibody in the eukaryotic expression plasmid EGFP-ICP22 transfection group (left panel) or DEV infection group (right panel).