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. 2020 Jan 17;9:463. doi: 10.3389/fcimb.2019.00463

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Copyright © 2020 Li, Wu, Wang, Ma, Jia, Chen, Zhu, Liu, Yang, Zhao, Zhang, Huang, Ou, Mao, Zhang, Liu, Yu, Pan, Tian, Rehman, Chen and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular localization of ICP22 and viral titres of one-step and multi-step replication kinetics. (A) Intracellular localization of ICP22 in infected DEFs at different time points with 10 MOI BAC-ΔUS1, BAC-ΔUS1 R309A, and BAC-ΔUS1 308-312AA. (B) Viral titer in the cytoplasm, supernatant, and total of one-step growth assays (top panel) and multi-step replication kinetics (bottom panel). Each time point was measured in triplicate, with the standard error indicated. A representative experiment with at least three repeats was performed. Ns represents no significance, *p < 0.1, **p < 0.01, ***p < 0.001 and ****p < 0.0001.