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. 2020 Jan 17;9:463. doi: 10.3389/fcimb.2019.00463

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Copyright © 2020 Li, Wu, Wang, Ma, Jia, Chen, Zhu, Liu, Yang, Zhao, Zhang, Huang, Ou, Mao, Zhang, Liu, Yu, Pan, Tian, Rehman, Chen and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of DEV CHv-BAC-ΔUS1 and DEV CHv-BAC-2ΔUS1. (A) Schematic diagram of constructing the duplicated US1 deletion mutant using the Red recombinant system. (B) DEV CHv-BAC-ΔUS1 and DEV CHv-BAC-2ΔUS1 were rescued in DEF cells.