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. 2020 Jan 16;4:119. Originally published 2019 Aug 9. [Version 2] doi: 10.12688/wellcomeopenres.15232.2

Figure 4. Localisation study for new regulators of glycoprotein secretion.

Figure 4.

Fluorescence microscopy images of HeLa cells expressing a FLAG-tagged cDNA construct show each protein’s localisation by co-staining with either an ER marker, calnexin, or a Golgi marker, TGN46. In the merged images, FLAG staining is shown in green and calnexin or TGN46 staining is shown in red. The subcellular localisation of each protein construct is indicated in green on the cell diagrams. Darker shades of green are used to indicate the most prominent location if the construct localises to more than one compartment. A– E show expression of FLAG-tagged GPR161 ( A), TMEM220 ( B), MXRA7 ( C), FAM98B ( D) and FAM102B ( E). Original magnification 20x.