TABLE 3.
Supporting information for phylotype specific qPCR assays.
| Target mcrA gene | Annealing temperature/time | Average efficiency | R2 | Clone used | Mass of one gene (Da) | Primer sequences (5′ - 3′) | References | Primer concen- tration | Product length (bp) |
| ANME-1 | 62°C/30 s | 84.45% | >0.99 | E-3 | 878414 | F: AYGACCAGYTGT GGTTCGGAACGT | Miyazaki et al., 2009 | 600 nM | 175 bp |
| R: TCCATGTTSARC TTGTCGCCCTTY | |||||||||
| ANME-2a | 62°C/30 s | 84.96% | > 0.99 | F-79 | 862362 | F: ATATGGCAGATATTG TCCAGACCTCAAGG | 600 nM | 218 bp | |
| R: ATTTATCCC AKCCGTAYTC | |||||||||
| ANME-3 | 52°C/20 s | 85.67% | >0.984 | AII58 | 440445 | F: AAGGAYATYRS AACCGAATC | 400 nM | 180 bp | |
| R: TTGAAAGGTACC ATSSKGAAAGACC | |||||||||
| ANME-1- related | 52°C/20 s | 89.08% | >0.99 | E-155 | 870491 | F: GAGATCGCVRTV GACATGTTCGG | Zhou et al., 2014 | 400 nM | 172 bp |
| R: GCCCTMACAG AMCCRCCGAAGTG |