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. Author manuscript; available in PMC: 2020 Mar 18.
Published in final edited form as: ACS Appl Bio Mater. 2019 Jan 25;2(3):1050–1055. doi: 10.1021/acsabm.8b00654

Figure 1.

Figure 1.

(a) Schematic of the one-cell type BBB model. (b) Microscopy image of the brain endothelial cells grew on the transwell membrane after day 13. (c) Immunofluorescence of membrane proteins (Claudin-5) for one-cell type BBB model. Scale bar 20 μm. (d) Schematic of the three-cell type BBB model. (e) Photograph of a three-cell type BBB model. Reproduced with permission from Ref.16. Copyright 2017, American Chemical Society. (f) Immunofluorescence of membrane proteins (Claudin-5) on three-cell type BBB model. Scale bar 20 μm.