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A qRT-PCR analysis was performed for young and aged particle-challenged mBMMs. The mRNA expressions of (a-c) iNOS and (d-f) CCL2 were assessed at 6 h, 12 h, and 24 h with M0, M1, and M2 macrophage phenotypes included. * represents significant difference between similarly treated young and aged macrophages, while ‡ indicates statistical significance of particle stimulation.
