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Using a lentiviral vector-based luciferase reporter system, the activity of transcription factor NF-κB was analyzed from (a) young and (b) aged particle-stimulated mBMMs of M0, M1, and M2 phenotype. The luciferase activity was measured 4 h after the initiation of particle challenge, and normalized to the amount of total protein. Data are presented as fold change compared to untreated M0 macrophages. * stands for statistically significant difference between indicated phenotypes.
