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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2019 Jul 20;15(3):390–399. doi: 10.1007/s11481-019-09864-z

Figure 1. Knockdown of lincRNA-Cox2 reverses the expression of LPS-induced cell cycle genes in microglia.

Figure 1.

(A) Heat map analysis of microarray data showing hierarchical clustering of lincRNA-Cox2-regulated cell cycle genes in LPS stimulated BV-2 cells. (B) Mouse primary microglial cells were transfected with lincRNA-Cox2-siRNA for 24 h, followed by exposure of cells to LPS for 4 h, and subsequently assessed for the expression of lincRNA-Cox2 by qPCR, and relative gene expression was calculated from Ct values using a 2^ΔΔCt method. (C) - (I) Validation of representative microarray data by qPCR for genes indicated in mouse primary microglia (Also see (B)). The relative amounts of mRNA for each of the indicated genes were determined by qPCR, and relative gene expression was calculated from Ct values using a 2^ΔΔCt method. Fold-changes were normalized to control siRNA of untreated sample. Data are mean ± SEM of at least three independent experiments. Nonparametric Kruskal-Wallis one-way ANOVA followed by Dunn’s post hoc test was used to determine the statistical significance between multiple groups. *p < 0.05; * *p < 0.01, * * *p < 0.001 versus control. #p < 0.05, # #p < 0.01, # # #p < 0.001 versus control-siRNA + LPS group.