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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2019 Jul 20;15(3):390–399. doi: 10.1007/s11481-019-09864-z

Figure 3. EVs can be used for the delivery of siRNA.

Figure 3.

(A) EVs were characterized by western blot for exosomal markers —Alix, CD63, TSG101. Calnexin (non-exosomal protein) was used as negative control. (B) Representative transmission electron microscopy (TEM) observation of EVs, Scale bar= 100nm. (C) Size and particle distribution plots of isolated EVs from cell culture by ZetaView nanosight tracking analysis (NTA). EVs are visualized in the inset image - a screenshot of a video generated from the ZetaView NTA showing light scattering of EVs. (D) EVs loaded with Cy5 labeled small RNA were taken up by mouse primary microglia. (E) Mouse primary microglial cells were treated with lincRNA-Cox2-siRNA loaded EVs for 24h followed by assessment for the expression of lincRNA-Cox2 by qPCR. Data are mean ± SEM of six independent experiments. Significant differences (two-tailed t-test), ****p < 0.0001; ns, not significant.