Table 3. Different analytical approaches commonly used to determine cholesterol in biological samples.

The type of detection and the limit of quantification (LOQ) of each methodology are shown.

Method	Detection	LOQ (ng/μL)
Enzymatic/colorimetric [26]	500 nm (quinoneimine)	250
Enzymatic/fluorometric [27]	585 nm (resorufin)	0.1
HPLC-UV [present work]	205 nm (cholesterol)	62.5
LC-MS	m/z	< 50
GC-MS	m/z	< 50