Table 3. Germline deletion of Surf4 results in lethality between embryonic day 3.5 and 9.5.
Timed matings were performed between Surf4+/- mice carrying the del(1) allele and embryos harvested at E9.5, E12.5, E.14.5 or at the time of weaning. For analysis at E3.5, blastocysts were collected following in vitro fertilization of oocytes from Surf4+/- females with sperm from Surf4+/- males. The proportion of mice with the homozygous null genotype was compared to expected Mendelian ratios by the chi-square test.
| Surf4+/- x Surf4+/- | |||||
|---|---|---|---|---|---|
| Allele | Stage | Progeny | p (Χ2) | ||
| +/+ | +/- | -/- | |||
| del (1) | weaning | 40 | 72 | 0 | <0.01 |
| E14.5 | 2 | 3 | 0 | >0.99 | |
| E12.5 | 3 | 12 | 0 | 0.100 | |
| E9.5 | 5 | 15 | 0 | 0.047 | |
| E3.5 | 1 | 7 | 4 | >0.99 | |