Fig 1. K05 affects both growing and stationary E. coli cultures in a MscL-dependent manner.
(A) percentage of growth (OD600), normalized to untreated samples, for K05 treated (red) relative to non-treated (blue) in Eco-MscL-expressing cultures; the ΔmscL is shown in black. (n = 3–4) (B) The percent reduction in CFU’s after treatment with 80 μM of compound K05 after cultures have reached stationary phase. (n = 4) *** p<0.0004 by unpaired Student t-test. (C) Representative traces of Eco-MscL channels recorded by patch clamp experiments of native membranes. MscL channel activity is shown before (upper trace) and after (lower trace) addition of 50μM compound K05 to the bath. (D) Quantification of the effect of K05 on the probability of opening of MscL (NPo). (n = 6) p<0.02 paired Student t-test.