Fig 4. K05 affects both growing and stationary cultures of pathogenic bacterial models in a MscL-dependent manner.

Growth inhibition by increasing concentrations of compound K05 are shown for (A) S. aureus R4220 WT (red) and ΔmscL R4220 S. aureus (grey) strains and (B). M. smegmatis MC2155 WT (green) and MC2155 ΔmscL (grey). Curves represent the percentage growth (OD₆₀₀) of K05 treated, relative to untreated. Values represent the mean SEM of at least three experiments n = 3 (C). Viability of stationary phase cultures after treatment with 80 μM of compound K05. Values represent the percent reduction in CFU’s normalized by the untreated shown for the S. aureus R4220 WT and null (red) and M. smegmatis MC2155 WT and null (green) Note that WT stains are expressing endogenous levels of MscL. *p<0.03 (n = 4); ***p<1.5 10⁻⁵ (n = 7) Student t-test unpaired.