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. 2020 Jan 24;15(1):e0227994. doi: 10.1371/journal.pone.0227994

Fig 3. Summary of predicted gRNA rankings vs. observed percent reduction in luminescence of NanoLuc, measured 4 dpi in three biological replicates.

Fig 3

NanoLuc luminescence measured in leaves infiltrated with p2X35S-NanoLuc-NOSt, p19 and a NanoLuc editing construct. Percentage reduction of luminescence due to editing of NanoLuc, calculated by setting luminescence of control leaves (infiltrated with p2X35S-NanoLuc-NOSt, p19 and an editing construct targeting an endogene in N.benthamiana) to 100%. See S1 Data for luminescence measurements. Each gRNA ranking obtained from the online gRNA prediction tools is colour coded: red, orange and green depict low, medium and high editing efficiency, respectively.