Fig 4.

Effect of shRNA mediated ACAT-1 inhibition on ovarian cancer cell proliferation, colony formation, migration and invasion properties (A) Cell proliferation assay of ovarian cancer cell lines stably transfected with control shRNA or ACAT-1 specific shRNA at 1, 2, 3, 4, 6, and 14 days post-seeding. Data are shown as mean ± SD of triplicate experiments. *P < 0.05, compared to the respective scrambled controls, Student’s t-test. (B) Left panel: Colony formation assay. Cells seeded at 200 cells/100 mm² petri dish were incubated for 21 to 28 days. Surviving colonies were fixed, stained with crystal violet and observed under microscope. Right panel: Quantitation of number of colonies. Data were expressed as mean ± SD of triplicate experiments. *P < 0.05, as compared to respective scrambled controls, Student’s t-test (C) Cell migration abilities of control shRNA transfected cell lines vs. ACAT-1 shRNA transfected cell lines. (D) Cell invasion assay on control sh-RNA vs. ACAT-1 shRNA transfected cells. Data represent mean ± SD of triplicate experiments. *P < 0.05, as compared to respective scrambled controls, Student’s t-test.