Fig 6. Bacterial diversity in healing and non-healing DFU microbiomes.

Diversity of the DFU microbiomes was compared in the samples collected at the initial visit, grouping the samples based on the healing outcome. The alpha diversity indices showing sample richness (richness), Shannon-Weaver diversity (diversity) and Pielou’s evenness (evenness) are presented based on healing outcome for (A) wound bed and (B) wound edge samples. (C) The compositional dissimilarity in the microbiome of healing and non-healing wounds is presented as a non-metric multidimensional scaling (NMDS) plot (goodness of fit or stress = 0.21) based on the ordination of Bray-Curtis dissimilarity (β-diversity). Direction and length of arrows indicate which GPAC contributed to the microbiota compositions of wounds that healed (Arthrobacter and Corynebacterium) or did not heal (Peptoniphilus, Anaerococcus, Finegoldia, and Helcococcus).