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. 2020 Jan 21;11(3):216–236. doi: 10.18632/oncotarget.27400

Figure 11. Short-hairpin (sh) modifier screen applied to NCI-H520R to identify genes capable of sensitizing cells to prexasertib treatment upon knockdown.

Figure 11

(A) Rank order of genes with greater than 50% effect on cell viability of the resistant tumor line induced by on-target knockdown normalized to non-target control (calculated from the geometric mean of short-hairpins; Supplementary Table 13). (B) Tertiary follow-up screen centered on 15 hits identified in the primary screen; y-axis corresponds to the percent effect on cell viability (as measured by the CTG assay) of on-target/non-target control established for DMSO (grey bar) or prexasertib-treated cells (black bar); red tracing depicts the ratio of DMSO/drug treatment effect. Concentration-response (10-point curve, ranging from 1 nM-1 μM) of NCI-H520 (C) and NCI-H520R cell viability (D) as a function of siRNA exposure.