Figure 13. shRNA modifier screen applied to the NCI-H520 cell line identified genes that induced a sensitizing or de-sensitizing effect upon knockdown.

(A) Y-axis corresponds to the ratio of the geometric means for prexasertib over DMSO. One corresponds to no effect, >1 shows hits that de-sensitize and <1 hits that sensitize to prexasertib upon knockdown. Hits from primary screen are shown in Supplementary Table 14. Confirmation of selected hits from shRNA screen using siRNA knockdown experiments in NCI-H520 (B) and the ovarian cancer cell line OVCAR3 (C).