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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Neurobiol Dis. 2019 Oct 9;134:104615. doi: 10.1016/j.nbd.2019.104615

Figure 1.

Figure 1.

Orexin-expressing neurons demonstrate increased cell size in hypothalamus of Tsc1GFAPCKO mice. A, Orexin-positive cells in hypothalamus were assessed by orexin A (green) immunohistochemistry, followed by counterstaining with TO-PRO-3 Iodide (blue) for the nonspecific nuclear staining of all cells. B, Four-week-old Tsc1GFAPCKO mice (KO+Veh) had significantly increased orexin A positive cell size in the hypothalamus compared with control mice (Cont+Veh). Rapamycin treatment (3 mg/kg/day i.p. for one week starting at 3 weeks of age) significantly reduced the orexin A positive cell size of Tsc1GFAPCKO mice (KO+Rap) back to control levels. C, There was a trend toward increased orexin-A cell number in Tsc1GFAPCKO mice, but there was no statistically significant differences between groups in cell number. D, Mean intensity of orexin expression in hypothalamus was significantly increased in Tsc1GFAPCKO mice compared with control mice, and was reversed by rapamycin. * p<0.05, **p<0.01, *** p<0.001 by one-way ANOVA (n = 6-8 mice/group). Cont = control mice, KO = Tsc1GFAPCKO mice, Veh = vehicle, Rap = rapamycin. Scale bars = 100 μm (low power), 50 μm (high power inset).