Macrophage activation on PEG hydrogels is mediated by MyD88. A) Schematic of MyD88-dependent and MyD88-independent signaling by pre-adsorbed proteins. B) mRNA production of the pro-inflammatory cytokine genes Tnfa, Il6, and Il1b relative to the housekeeping gene, Rpl32 for WT (black bars) and MyD88−/− (gray bars) macrophages 4 h postseeding.
Data are presented as mean with standard deviation as error bars (n=4) and with pvalues from pairwise comparisons (* p<0.05; *** p<0.001).