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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Biochim Biophys Acta Gen Subj. 2019 Nov 15;1864(3):129482. doi: 10.1016/j.bbagen.2019.129482

Figure 3. PRMT1 knockout leads to tadpole lethality.

Figure 3.

(A) Representative photos of 4-day-old tadpoles. Ventral view of a wild-type (upper panel) and PRMT1 homozygous knockout (lower panel) tadpole. Bars: 1 mm.

(B) Genotype distribution of tadpoles at the age of 4, 6, 8, 10, 12 and 14 days. 30 tadpoles were randomly selected and genotyped by PCR (Figure 1C) at indicated age (days). Note that no homozygous knockout animals were found at day 14.

(C) PRMT1 knockout leads to lethality within two weeks after fertilization. Tadpoles were genotyped at 4 days of age and the survival rate of wild-type (N=35), heterozygous (PRMT1(+/−), N=83) and homozygous (PRMT1(+/−), N=32) was determined from the age of day 4 to day 14. Note that PRMT1(+/−) tadpoles started to die around day 12 and completely died by day 14.