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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Neurobiol Dis. 2019 Oct 17;134:104636. doi: 10.1016/j.nbd.2019.104636

Fig. 2.

Fig. 2.

AV-1980R/A formulated in AdvaxCpG adjuvant generated both Th1 and Th2 immune responses in Tg4510 mice. Concentrations (pg/ml) of Th1 cytokines (A, B, C) and Th2 cytokines (D, E) in response to AdvaxCpG adjuvant (open circle) and AV-1980R/A tau vaccine (black square) in Tg4510 mice. [IFN-γ: Interferon-gamma, IL-2: Interleukin 2, TNF-α: Tumor necrosis factor alpha, IL-4: Interleukin 4, IL-5: Interleukin 5]. Cytokines were detected using Mouse Th1/Th2 Cytokine CBA kit by flow cytometry. Splenocytes were re-stimulated in vitro with a cocktail of 12 Th epitope peptides (2 μg/ml each) for 24 h and supernatant was collected for assay. Bars represent mean ± SEM (n = 6 mice/per group). Statistical significance was calculated against AdvaxCpG group using unpaired t-test. (*P < 0.05, ***P < 0.001 and ****P < 0.0001).