Fig. 5.

Vaccination with AV-1980R/A did not improve spatial memory during radial arm water maze testing but rescued the short-term memory deficit observed in Tg4510 during the novel object recognition test.

(A) Tg4510 mice made significantly more errors regardless of treatment with Advax^CpG adjuvant or AV-1980R/A when attempting to locate a hidden platform during the 2-day radial arm water maze (RAWM) test when compared to non-transgenic (Ntg) and tTA littermates. Data are presented as mean ± S.E.M., n = 12/group. ***p < 0.001. (B) Tg4510 mice treated with Advax^CpG adjuvant spent less time with novel object compared to non-transgenic (Ntg) and tTA littermates as they displayed a lower discrimination ratio (which is defined as the time exploring the novel object minus the time spent with familiar and divided by combined time spent exploring both novel and familiar objects), indicating short term memory deficit. Tg4510 mice vaccinated with AV-1980R/A displayed a significant increase in discrimination ratio compared to control Tg4510 mice treated with Advax^CpG adjuvant indicating an improvement in short memory. Data are presented as mean ± S.E.M., n = 12/group. *p < 0.05. 2-way ANOVA test was used for RAWM with Tukey’s LSD means comparisons for significant overall effects. A t-test was performed to compare the two Tg4510 groups for NOR.