Fig. 6.

Tg4510 mice vaccinated with Tau vaccine AV-1980R/A displayed increased levels of mouse IgG in the brain compared to Advax^CpG

(A) Quantification of positive area stained for mouse IgG in anterior cortex (ACX), hippocampus (HPC) and posterior cortex (PCX) in Tg4510 treated with Advax^CpG adjuvant or with AV-1980R/A mice. Non-transgenic and tTA littermates displayed similar levels to Tg4510 treated with Advax^CpG adjuvant (data not shown). Immunostaining quantification was performed utilizing Mirax software (Zeiss Inc.). (B) Micrographic representation of Mouse IgG immunostaining in the posterior cortex of Tg4510 mice treated with Advax^CpG adjuvant (a, c) or AV-1980R/A (b, d). c and d are magnification of square area in a, b, respectively. Tg4510 mice immunized with AV-1980R/A seemed to display positive staining in cell bodies particularly in the posterior cortex. Data are presented as mean ± S.E.M., n = 12/group. Statistical significance was calculated against Advax^CpG group using unpaired t-test *p < 0.05.