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. Author manuscript; available in PMC: 2020 Jan 24.
Published in final edited form as: Cell Rep. 2019 Dec 17;29(12):3902–3915.e8. doi: 10.1016/j.celrep.2019.11.039

Figure 6. Deletion Analysis of ELF1e DIVAC Element.

Figure 6.

(A) Hi-C and DIVAC-trap HTISA data for the ELF1 locus, presented as in Figure 3. Red arrow, location of ELF1e element.

(B) GFP fluorescence loss (3 weeks of culture) in Ramos clones infected with GFP7 containing no-DIVAC, ELF1e, or truncation mutants of ELF1e. Data are presented as in Figure 5A. Data points outside of the y axis range are in parentheses.

(C) Diagram of entire ELF1e element with tracks for NIPBL, H3K27Ac, H3K4me1, H3K4me3, and GRO-seq. Locations of binding motifs for transcription factors (Z, ZEB1; P, PU.1; M, MEF2; E, E2A; B, BCL6; Y, YY1) are marked. The region of ELF1e retained in the mutants in (B) or deleted from the mutants in (D) is indicated with color-coded bars.

(D) Analysis as in (B), but using GFP7 vectors in which small regions were deleted from full-length ELF1e. Deleted regions 1–3 are indicated in (C). Data points outside the y axis range are in parentheses.