Effects of IAA exposure on gene expression of Ccna2, Ccne1, Ccnb1, Ccnd2, Cdk4, and Cdkn1a, in follicles treated with 2, 5, 10 and 15 μM of IAA. RNA was extracted from snap-frozen follicles, reversed transcribed to cDNA, and subjected to quantitative real time PCR. Graphs represent means ± SEM from 3 separate cultures. Asterisks (*) indicate significant differences compared to control (P ≤ 0.05).