Effects of IAA exposure on gene expression of Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd17b, and Cyp19a1 in follicles treated with 2, 5, 10 and 15 μM of IAA. RNA was extracted from snap-frozen follicles, reversed transcribed to cDNA, and subjected to quantitative real time PCR. Graphs represent means ± SEM from 3 separate cultures. Asterisks (*) indicate significant differences compared to control (P ≤ 0.05).