Table 1.
Primers used in quantitative real-time polymerase chain reactions (qPCR)
| Gene | Gene Symbol | Primer Sequence | |
|---|---|---|---|
| Forward | Reverse | ||
| Beta actin | Actb | GGGCACAGTGTGGGTGAC | CTGGCACCACACCTTCTAC |
| Steroidogenic acute regulatory protein | StAR | CAGGGAGAGGTGGCTATGCA | CCGTGTCTTTTCCAATCCTCTG |
| Cytochrome P450 cholesterol side-chain cleavage | Cyp11a1 | AGATCCCTTCCCCTGGCGACAATG | CGCATGAGAAGAGTATCGACGCATC |
| 3b-Hydroxysteroid dehydrogenase 1 | Hsd3b1 | CAGGAGAAAGAACTGCAGGAGGTC | GCACACTTGCTTGAACACAGGC |
| Cytochrome P450 steroid 17-a-hydroxylase 1 | Cyp17a1 | CCAGGACCCAAGTGTGTTCT | CCTGATACGAAGCACTTCTCG |
| 17b-Hydroxysteroid dehydrogenase 1 | Hsd17b1 | ACTGTGCCAGCAAGTTTGCG | AAGCGGTTCGTGGAGAAGTAG |
| Cytochrome P450 aromatase | Cyp19a1 | CATGGTCCCGCAAACTGTGA | GTAGTAGTTGCAGGCACTTC |
| Estrogen receptor 1 (alpha) | Esr1 | CCGTGTGCAATGACTATGCC | GTGCTTCAACATTCTCCCTCCTC |
| Estrogen receptor 2 (beta) | Esr2 | GGAATCTCTTCCCAGCAGCA | GGGACCACATTTTTGCACTT |
| Cyclin A2 | Ccna2 | GCTCTACTGCCCGGAGGCTGA | TGGCCTACATGTCCTCTGGGGAA |
| Cyclin B1 | Ccnb1 | TGCATTCTCTCAGTGCCCTCCACA | AGACAGGAGTGGCGCCTTGGT |
| Cyclin D2 | Ccnd2 | CCTTTGACGCAGGCTCCCTTCT | ACCCTGGTGCACGCATGCAAA |
| Cyclin E1 | Ccne1 | GGTGTCCTCGCTGCTTCTGCTT | CCGGCTAACCATGGCGAACGGA |
| Cyclin-dependent kinase 4 | Cdk4 | AGAAACCCTCGCTGAAGCGGCA | TGGGGGTGAACCTCGTAAGGAGA |
| Cyclin-dependent kinase inhibitor 1A (p21) | Cdkn1a | TTAGGCAGCTCCAGTGGCAACC | ACCCCCACCACCACACACCATA |
| Proliferation marker protein Ki-67 | Ki67 | GCTCACCTGGTCACCATCAA | ACTACAGGCAGCTGGATACG |
| Proliferating cell nuclear antigen | Pcna | CGGCGTGAACCTGCAGAGCA | GGTTGCGGTCGCAGCGGTAT |
| B cell lymphoma 2 | Bcl2 | ATGCCTTTGTGGAACTATATGGC | GGTATGCACCCAGAGTGATGC |
| Bcl2 like protein 10 | Bcl2l10 | CGCTACACACACTGACTGGA | CTTTAGGATCCCCTGCCCTG |
| Bcl2-associated X protein | Bax | TGAAGACAGGGGCCTTTTTG | AATTCGCCGGAGACACTCG |
| Apoptosis-inducing factor 1, mitochondrion-associated 1 | Aimf1 | AGGACGGTGAGCAACATGAA | GTTCTATCCACCCATCCCGC |