The endocannabinoid system comprises of cannabinoid receptor 1 and 2 (CB1R, CB2R), their endogenous ligands 2-arachidonoylglycerol (2-AG) and anandamide (AEA) and their metabolic enzymes: diacylglycerol lipase α and β (DAGLα, DAGLβ), monoacylglycerol lipase (MGLL), α,β-hydrolase domain containing proteins 6 and 12 (ABHD6, ABHD12), N-acylphosphatidylethanolamine phospholipase D (NAPE-PLD) and fatty acid amide hydrolase (FAAH). Alternative multi-step pathways for AEA biosynthesis are not shown.