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. Author manuscript; available in PMC: 2020 Jan 24.
Published in final edited form as: Cell Rep. 2019 Dec 3;29(10):3331–3348.e7. doi: 10.1016/j.celrep.2019.10.117

Figure 6. AMPK Phosphorylates Stim1 and Stim2 in Response to Metformin in Mouse Liver.

Figure 6.

(A) Heatmap of Gene Ontology process: calcium ion binding, purple arrows indicate STIM1 and STIM2.

(B) Recombinant 14-3-3-GST or GST pull-downs in AMPK WT or AMPK DKO liver homogenates in biological duplicate for each biological condition.

(C) Co-immunoprecipitation in mouse embryonic fibroblasts either WT or AMPK DKO for STIM2 and AMPKα using two different polyclonal antibodies against Stim2 full-length sequence.

(D) Primary mouse hepatocytes from wild-type or AMPK DKO mice extracted and treated with a dose-response curve of 991 at 0, 10, 25, and 50 μM and compared to 2 mM phenformin for 60 min.

(E) AMPK WT and DKO mice treated with 30 mg/kg MK8722 for 4 h, and liver homogenates analyzed by western blot analysis.