FIG. 4.
Current systems for deposition, archiving, and accessing cryo-EM structures. Worldwide, every cryo-EM structure (map, experimental metadata, and optionally coordinate model) is deposited and processed through the wwPDB OneDep system (deposit.wwpdb.org), following the same annotation and validation workflow also used for X-ray crystallography and NMR structures.17,18 Map-only depositions yield an EMDB entry, while joint map+model depositions yield both EMDB and PDB entries. Workflow metadata collected in OneDep are passed to both EMDB and PDB. EMDB holds all workflow metadata while PDB holds a subset of the metadata; see Table I. The PDB and EMDB archives are accessible by FTP and rsync at wwPDB mirror sites in the US, UK, and Japan. Released cryo-EM structure data from both archives can be accessed via EMDataResource, EMDB, and wwPDB partner websites.