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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Neurochem. 2019 Aug 22;152(3):381–396. doi: 10.1111/jnc.14834

Figure 6. Tim-2 expression in oligodendrocytes, microglia, and astrocytes.

Figure 6

Slides were stained for Tim-2 (magenta) or CC1/Iba1/GFAP (yellow); nuclei were counterstained with DAPI (cyan). A) Mouse corpus callosum stained for CC1 (yellow), a marker for myelinating oligodendrocytes, and Tim-2 (magenta) reveal expression of Tim-2 in oligodendrocytes. There is similarly robust Tim-2 staining in CC1-negative cells, likely to be neurons. B) Dual-staining in the neocortex for Iba1 and Tim-2 reveals microglia (Iba1+, white arrows) do not express Tim-2. C) Hippocampus staining for GFAP (yellow), an astrocytic marker, demonstrates colocalization with Tim-2 (magenta), marked by white arrowheads. The white arrow marks cells surrounding a blood vessel positive for Tim-2 staining, likely to be endothelial cells of the BBB. There can again be seen robust staining for Tim-2 in non-GFAP+ cells, most likely to be neurons. All brain slices came from wild-type PND7 female mice. Scale bars indicate 10 µm.