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. Author manuscript; available in PMC: 2020 Jul 20.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Dec 3;59(4):1465–1468. doi: 10.1002/anie.201910960

Figure 3.

Figure 3.

The release of 15NH4+ ions upon association of Antp HD to the 15-bp DNA duplex. (A) NMR signal intensity modulation as a function of the strengths of the magnetic field gradient for solutions containing 1.4 mM 15-bp DNA in the presence (magenta) and absence (black) of 1.15 mM Antp HD. Uncertainty in signal intensity is smaller than each symbol. (B) Differences in measured diffusion coefficients. Data are compared for 1) the 1.4-mM DNA solution vs. the buffer, 2) the 1.4-mM DNA solution vs. the 1.4-mM DNA + 1.15-mM Antp HD solution; 3) the 1.4-mM DNA solution vs. the 1.4-mM DNA + 1.15-mM BPTI solution and 4) the 1.4-mM DNA solution vs. the 1.4-mM DNA + 1.15-mM ubiquitin solution.