(A) Albuminuria was not impacted by diet (2-way ANOVA, diet effect: P=0.43) but similarly increased in both groups over time (time effect: P< 0.0001; Sidak’s test of week 28 vs. week 4: P<0.05). (B) UACR was not different after 28 weeks on diet (t-test, P=0.90). (C) Expression levels of TGF-β, fibronectin, collagen I, and α-SMA measured by Western blot in whole kidney samples were not different between experimental groups (t-test, P>0.05). NFD, normal fat diet (withe); HFD, high fat diet (black); Data are mean ± SEM.