After 28 weeks on the diet, HFD mice showed a significant elevation in (A) urinary excretion rate of uAGT (2-way ANOVA, diet effect: P<0.001; time effect: P<0.001; Sidak’s test of week 28 vs. week P<0.001). In addition, HFD increased (B) kidney Ang II levels (t-test, P=0.01) and (C) urine Ang II (t-test, P=0.02). NFD, normal fat diet (withe); HFD, high fat diet (black); Data are mean ± SEM.