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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Immunol. 2019 Dec 30;204(3):586–595. doi: 10.4049/jimmunol.1900677

Figure 2. Comparison of kidney & spleen CD4+ T cells from WT or NGAL KO mice.

Figure 2.

Kidney mononuclear cells and splenocytes were obtained from WT and NGAL KO male mice and analyzed using flow cytometry. There was no difference in the absolute number of kidney/splenic CD4+ T cells between WT and NGAL KO groups. Kidney CD4+ T lymphocytes from NGAL KO mice had significantly lower number of Tregs and Ki-67+ proliferating cells compared to WT mice. Lcn2-deficient kidney CD4+ T cells also had reduced number of CD62L positive cells compared to kidney CD4+ T cells from WT mice. There was no difference in the number of CD69 or CD44 positive CD4+ T cells. There were no differences in any of these parameters when comparing splenic CD4+ T cells from WT and NGAL KO mice (n=3). Data presented as mean±SE. *P < 0.05, **P < 0.01, ***P < 0.001.